Fig. 5

Sensitive detection of pathological and seeding-competent Tau forms in AD (Braak V-VI) and non-AD (Braak I-II) hippocampus. a Tau-SAA time to threshold (TTT) values of AD (Braak V-VI) and non-AD (Braak I-II) hippocampus (Hip) and cerebellum (Cer) homogenates (see Additional file 1: Table S3), diluted by 10^–6. Dots indicate medians of technical quadruplicates per brain; crosses: non-seeding samples with at least 3 out of 4 technical replicates showing TTT values ≥ 200 h. b-f Immunoassay results for aggregated Tau, sarkosyl-insoluble Tau, pTau212/214, pTau181, and total Tau. Dots: mean of technical duplicates. Dotted lines indicate the limit of detection (LOD). Bars: group medians + interquartile range. Statistical comparisons of group medians were performed by Mann–Whitney test. *p < 0.05, **p < 0.01, ***p < 0.001. g–k. Spearman correlation of Tau-SAA TTT versus immunoassay results. Non-seeding samples and values below the LOD were excluded. Cohort 2 is described in detail in Table 2 and Additional file 1: Table S3