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Fig. 2 | Acta Neuropathologica Communications

Fig. 2

From: Tau seed amplification assay reveals relationship between seeding and pathological forms of tau in Alzheimer’s disease brain

Fig. 2

Tau SAA detected seeding by AD brain homogenate and recombinant Tau fibrils with very high sensitivity. a Illustration of the six Tau isoforms in the human brain with amino-terminal domains N1 and N2 and repeat domains R1-R4. Amino acid numbering on the top is referring to the 2N4R isoform. Arrow indicates the 0N3R Tau isoform used in Tau SAA. b Thioflavin T (ThT) kinetic curves of serially diluted AD (red) vs. control (CTR, blue) brain pools. Dotted lines depict the threshold of 1500 relative fluorescent units (rfu) for determination of the time to threshold (TTT) value. Curves are medians of technical quadruplicates. Numbering indicates dilution factor. c TTT values derived from curves shown in b. Each dot depicts a technical replicate, bars are medians, and error bars show the interquartile range. RB: reaction buffer. d–e. Tau SAA results of 2N3R Tau (green) fibrils. f–g Tau SAA results of 2N4R (orange) Tau fibrils

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Acta Neuropathologica Communications

ISSN: 2051-5960

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