Fig. 7

EMP3 silencing synergizes with CDK2 inhibition in EGFR-amplified NCH1425 GSCs. A Western blots verifying successful EMP3 silencing in doxycycline-treated NCH1425 GSCs transduced with inducible EMP3 shRNAs. B Fold-change in the viability of NCH1425 GSCs after induction of shRNA expression and treatment with increasing concentrations of the CDK2 inhibitor K03861 (multiple Welch’s t-test; **P < 0.01, ****P < 0.0001). C Volcano plots showing differentially expressed genes upon doxycycline-induction of EMP3 shRNA expression in NCH1425 cells. Genes that were upregulated and downregulated upon EMP3 silencing are colored red and green, respectively. D STRING network of downregulated genes in NCH1425 expressing the EMP3 shRNA. For simplicity, nodes not belonging to the main cluster were removed from the network. Node borders are colored according to Reactome and Pfam protein domain terms. E Top 5 inhibited MRs based on the 85 DEGs unique to NCH1425 expressing the EMP3 shRNA. MRs putatively regulating the input genes are listed on the y-axis and ordered according to significance. Circle sizes represent the number of associated causal networks (CNs) per MR, while the color scale indicates the activation z-score of each MR (red—active; blue—inactive)