Fig. 1

BioID2-based proximity labeling of EMP3 WT and N47A in U-118 glioblastoma cells. A Venn diagram showing the number and extent of overlap of identified EMP3-proximal proteins between all pairwise comparisons. B Heat map showing the percentage of EMP3-proximal proteins with localization scores ≥ 4 in each cellular compartment and for each pairwise comparison. C STRING interaction network resulting from the union of hits identified with EMP3 WT as bait and TagRFP or GAP-TagRFP as spatial reference controls. Edges corresponding to STRING scores > 0.700 and nodes with degrees ≥ 3 were included in the network. Nodes were clustered into functional groups based on STRING enrichment analysis. For simplicity, nodes not belonging to any cluster were removed from the network. Node borders are colored according to plasma membrane localization score, while edge thickness corresponds to STRING interaction scores