Fig. 7

Emergence of vascular-associated microglia in chronic hypertensive states (a) Immunofluorescence confocal microscope image of microglia stained with anti-IBA1 representative from hippocampal CA1 region of late hypertensive rats (lower) and its age-matched normotensive control (upper). (b) Frequency of vascular-associated microglia (VAMs) in the hippocampal CA1 region. (c) Immunofluorescence microglia staining representative from retrosplenial cortex with anti-IBA1 of late hypertensive rats (lower) and its age-matched normotensive control (upper) (d) Frequency of VAMs in the retrosplenial cortex. HTN, Hypertension; IBA1, ionized calcium-binding adapter molecule 1 (microglial marker); STL, solanum tuberosum lectin-fluorescein isothiocyanate (endothelial marker). p-values: * ≤ 0.05; ** for p ≤ 0.01; *** for p ≤ 0.001; **** for p ≤ 0.0001