Table 1 List of antibodies
Antibody | Species | Dilution | Company | Catalog no |
|---|---|---|---|---|
Acetylated p65 (acetyl K310) | Rabbit | 1:1000 | Abcam | ab19870 |
Alpha-bungarotoxin-647 conjugate | Mouse-Conjugated | 1:500 | Thermo scientific | B35450 |
Beta-Actin | Mouse | 1:30,000 | Cell Signaling Technology | 3700 |
CD11b | Rat | 1:200 | BioRad | MCA711 |
ChAT | Goat | 1:500 | Sigma-Aldrich | AB144p |
GFAP | Chicken | 1:500 | Sigma-Aldrich | AB5541 |
Human FUS | Rabbit | 1:500 | Produced by Yu lab [42] | N/A |
Iba1 | Rabbit | 1:500 | Wako | 019-19741 |
MAP2 | Chicken | 1:500 | Abcam | AB5392 |
MAP2 | Mouse | 1:500 | Sigma-Aldrich | MAB3418 |
NeuN | Mouse | 1:500 | Sigma-Aldrich | MAB377 |
Neurofilament light | Mouse | 1:1000 | Cell Signaling Technology | 2835 |
SV2 | Mouse | 1:1000 | DSHB | SV2 |
Synaptophysin | Mouse | 1:500 | Synaptic System GmbH | 101–011 |
TOM20 | Rabbit | 1:500 | Santa Cruz Biotechnology | sc-11415 |
Total FUS | Rabbit | 1:500 | Sigma-Aldrich | HPA008784 |
Alexa Fluor 488 goat anti-chicken | Chicken | 1:500 | Abcam | ab150169 |
Alexa Fluor 488 goat anti-mouse | Mouse | 1:500 | Life technologies | A11001 |
Alexa Fluor 488 goat anti-rabbit | Rabbit | 1:500 | Life technologies | A11034 |
Alexa Fluor 546 goat anti-mouse | Mouse | 1:500 | Life technologies | A11030 |
Alexa Fluor 546 goat anti-rabbit | Rabbit | 1:500 | Life technologies | A11035 |
Alexa Fluor 647 goat anti-mouse | Mouse | 1:500 | Life technologies | A21236 |
Alexa Fluor 647 goat anti-rabbit | Rabbit | 1:500 | Life technologies | A21245 |