Fig. 3
FUSR521G causes cell-autonomous defects in neuromorphology. a Neurolucida tracing of cortical motor neuron dendrites from 1-month-old mice. b Sholl analysis shows significant reductions in the dendritic intersections and cumulative area of dendrites in cortical motor neurons of 1-month-old hFUSR521G/Syn1 mice compared to littermate controls (CTL). c Golgi images of dendritic spines from layer IV-V neurons in the motor cortex from 1-month-old mice and d their quantification. The dendritic spine density of total and mature spines shows no differences between 1-month-old hFUSR521G/Syn1 and CTL mice. e Neurolucida tracing of spinal motor neuron dendrites from 1-month-old mice. f Sholl analysis shows no reduction in the dendritic intersections and cumulative area of dendrites in spinal motor neurons of 1-month-old hFUSR521G/Syn1 mice. g Neurolucida tracing of cortical motor neuron dendrites from 6-months-old mice. h Sholl analysis shows persistent and significant reductions in the dendritic intersections and cumulative area of dendrites in cortical motor neurons of 6-months-old FUS transgenic mice. i Golgi images of dendritic spines from the cortical motor cortex of 6-months-old mice and j their quantification. The mature dendritic spine density of 6-months-old hFUSR521G/Syn1 mice shows significant reduction in the number of mature spines. k Neurolucida tracing of the dendrites of spinal motor neurons from 6-months-old mice. l Sholl analysis shows significant reductions in the dendritic intersections and cumulative area of dendrites in spinal motor neurons of 6-months-old FUS transgenic mice. Values from each group are expressed as mean ± SEM. Statistics uses two-way repeat measures ANOVA for Sholl analysis and an unpaired Student’s t-test for comparison between two groups (n = 3–4 mice/group). *p < 0.05, **p < 0.01, ***p < 0.005 ****p < 0.001, and not significant (ns)