Fig. 4
From: Human tauopathy strains defined by phosphorylation in R1-R2 repeat domains of tau
Phospho-Plus mutation on Ser305 abrogate seeding by AD-tau. a–c. AD-tau ( +), PSP-tau ( +) or unseeded (–) HEK293T cells transfected with P301L, P301L/S305E tau or GFP were fractionated into detergent-soluble and detergent-insoluble lysates and probed for total tau (t-tau). Quantitation of % aggregation (insoluble/[soluble + insoluble]*100) for each variant are shown (c). 2-way ANOVA with Bonferroni’s test. ***p < 0.001; **** p < 0.0001