Fig. 3
From: Human tauopathy strains defined by phosphorylation in R1-R2 repeat domains of tau
Phosphorylation profile of MTBR-associated tau variants following seeding. a. Schematic depiction of Phospho-Plus Ser/Thr → Glu tau variants generated on human 0N/4R P301L backbone and antibody epitopes used to detect tau phosphorylation. b. Detergent-insoluble fraction of AD-tau ( +), PSP-tau ( +) or unseeded (–) HEK293T cells transfected with different tau variants and GFP were probed for different epitopes. Relative molecular masses are indicated on the left of each blot. Blots showing technical replicate and detergent-soluble fractions are presented in Additional file 1: Fig. S7 a-d