Fig. 6

Overexpression of Nrf2 in GCL neurons in WT mice preserves visual function and protects RGCs at 2 and 5 wks post-IOP elevation. A) Quantification of PhNR amplitudes in AAV2/2.eGFP and AAV2/2.Nrf2 groups at 2 wks post-IOP elevation, **p < 0.001. B) Quantification of VEP N1 amplitudes in AAV2/2.eGFP and AAV2/2.Nrf2 groups at 2 wks post-IOP elevation, showing no differences between any groups. C) Representative brightfield micrographs of AAV2/2.eGFP and AAV2/2.Nrf2 optic nerves at 2 wks post-IOP elevation or post-saline injection. Scale bar applies to all micrographs. Quantification of total (D) and degenerative (E) axons at 2 wks post-IOP elevation, showing no differences in any groups. F) Quantification of PhNR amplitude in AAV2/2.eGFP and AAV2/2.Nrf2 groups at 5 wks post-IOP elevation, *p < 0.05, ***p < 0.0001. G) Quantification of VEP N1 amplitudes in AAV2/2.eGFP and AAV2/2.Nrf2 groups at 5 wks post-IOP elevation, ***p < 0.0001. H) Representative brightfield micrographs of AAV2/2.eGFP and AAV2/2.Nrf2 optic nerves at 5 wks post-IOP elevation or post-saline injection. Scale bar applies to all micrographs. Arrows point to degenerative axons. Quantification of total (I) and degenerative (J) axons in all groups at 5 wks post-IOP elevation, *p < 0.05, **p < 0.001, ***p < 0.001