Fig. 5

Overexpression of Nrf2 in GCL neurons in WT mice increases endogenous antioxidant response of the retina at 2 and 5 weeks post-IOP elevation. Representative Western blots (A) and quantification (B) for NRF2 normalized to b-actin, *p < 0.05, **p < 0.001. C) Representative fluorescence micrographs of retina GCL from mice with elevated IOP and treated with either AAV2/2.eGFP or AAV2/2.Nrf2 and labeled with anti-NRF2 (red) and anti-NeuN (green). Representative fundus images (D) and quantification (E) of DHE fluorescence in all groups at 2 wks post-IOP elevation, ****p < 0.00001. F) Quantification of antioxidant gene transcription (Prdx6, Nrf2, Sod3, Sod2, Gpx1 and Txn1) shown as fold change over appropriate saline controls at 2 wks post-IOP elevation. Representative Western blots (G) and quantifications (H) for PRDX6, GPX1, and SOD3 all normalized to b-actin at 2 wks post-IOP elevation, *p < 0.05, **p < 0.001. I) Quantification of DHE fluorescence in both AAV2/2.eGFP and AAV2/2.Nrf2 mice at 5 wks post-IOP elevation in comparison to saline-injected controls, *p < 0.05. Representative western blots (J) and quantification (K) for PRDX6 normalized to b-actin at 5 wks post-IOP elevation, **p < 0.001