Fig. 4

Nrf2 overexpression in GCL neurons in Nrf2 KO mice decreases ROS, preserves visual function and optic nerve histology. A) Representative Western blot for NRF2 normalized to b–actin showing increased levels of NRF2 in AAV2/2.Nrf2 injected retinas in comparison to AAV2/2.eGFP injected controls. Representative fundus images (B) and quantification (C) of DHE fluorescence in all groups, *p < 0.05, **p < 0.001. Representative Western blots (D) and quantification (E) of PRDX6, GPX1 normalized to b–actin, *p < 0.05. Representative PhNR and VEP waveforms (F) and quantifications of amplitudes (G, H) at 2 weeks post-IOP elevation. I) Representative brightfield micrographs of optic nerves, arrows indicate degenerative axons. Scale bar applies to all micrographs. Quantification of total (J) and degenerative (K) axons in all groups. *p < 0.05, **p<0.001, ***p < 0.0001 and ****p < 0.00001