Fig. 2

Knockdown of Nrf2 in either RGCs or astrocytes decreases visual function and accelerates axon degeneration. A) Representative waveforms for PhNR and VEP N1 in all experimental groups. B) Quantification of PhNR amplitudes in all experimental groups. C) Quantification of VEP N1 amplitudes in all experimental groups, with AAV2/6m.Vim.Cre group having the lowest amplitude in comparison to all other groups. *p < 0.05. D) Representative brightfield micrographs of optic nerves at 2- and 5-wks post-saline injection or IOP elevation in all experimental groups, with black arrows indicating degenerative axons. Scale bar applies to all micrographs. E) and F) Quantification of total and degenerative axons at 2 weeks. G) and H) Quantification of total and degenerative axons at 5 weeks. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001