Fig. 1

Knockdown of Nrf2 in either RGCs or glia decreases the IOP-induced endogenous antioxidant response. A, B) Plasmid maps of pAAV2.Sncg.Cre and pAAV2.hVimentin.Cre, respectively. C) Representative fluorescence micrograph of retina from a mouse transduced with AAV2/2.Sncg.Cre.tdTomato (red) and labeled with anti-NeuN (green). D) Representative fluorescence micrograph of retina from a mouse transduced with AAV2/6m.Vim.Cre.tdTom (red) and labeled with anti-GFAP (green). Scale bar shown is 50 μm and applies to all micrographs. E) Representative confocal micrograph of a flat-mounted retina from a mouse transduced with AAV2/6m.Vim.mCherry and labeled with anti-GFAP (green). F) Quantification of Prdx6, Gpx1, Sod3 and Ho-1, all ARE-driven transcripts, at 2 weeks post-IOP elevation in both AAV2/2.Sncg.Cre and AAV2/6m.Vim.Cre treated groups. Fold change was compared to their respective controls, AAV2/2.Sncg.tdTom and AAV2/6m.Vim.tdTom, *p < 0.05. G) Representative Western blots for NRF2 and ARE-related proteins. H) Quantification for pNRF2 normalized to total NRF2. I-K) Quantifications for PRDX6 (I), GPX1 (J), and SOD3 (K) all normalized to b-actin, *p < 0.05, **p < 0.001