Fig. 4
From: Cryo-EM structures of tau filaments from the brains of mice transgenic for human mutant P301S Tau
Structure of P301S Tau filaments from the cerebral cortex of PS19 mice. a, Cross-section through the cryo-EM reconstructions, perpendicular to the helical axis and with a projected thickness of approximately one rung, are shown for the P301S Tau filaments from the cerebral cortex of PS19 mice aged 48 weeks. The cryo-EM map resolution (bottom left) and the percentages of a given filament type among the filaments in the datasets (top right) are shown. Scale bar, 5 nm. b, Sequence alignment of the microtubule-binding repeats (R1-R4) of Tau with the observed eight β-strand regions (β1-β8, arrows). Mutant serine residue at position 301 is highlighted in red. c, Sharpened high-resolution cryo-EM map of the P301S Tau filaments with the atomic model overlaid. Residues R1-R4 and the C-terminal domain are coloured purple, blue, green, yellow and orange, respectively. d, Schematic of the PS19 Tau filament fold. Negatively charged residues are shown in red, positively charged residues in blue, polar residues in green, non-polar residues in white, sulphur-containing residues in yellow, prolines in purple and glycines in pink. Thick connecting lines with arrowheads indicate β-strands (β1-β8). The additional density is shown in red