Fig. 2
From: Cryo-EM structures of tau filaments from the brains of mice transgenic for human mutant P301S Tau
Immunogold negative-stain electron microscopy of Tau filaments extracted from the central nervous system of 24-week-old Tg2541 mice. a, Diagram of 0N4R P301S tau showing the repeats (R1-R4) and the epitopes of antibodies BR133 (N-terminus), BR136 (R1), anti-4R (R2), BR135 (R3), TauC4 (R4) and BR134 (C-terminus). b, Immunogold negative-stain electron microscopy of Tau filaments from the brainstem with BR136, anti-4R, BR135, TauC4, BR133 and BR134. c, Table summarising results from immunogold negative-stain electron microscopy of Tau filaments from frontal cortex, brainstem and spinal cord. Tick marks indicate antibody decoration of filaments and crosses indicate that the antibodies did not decorate filaments. Filaments were decorated by BR133, TauC4 and BR134, but not by BR136, anti-4R and BR135. Scale bar, 100Â nm