Fig. 3

Lack of regeneration and acquisition of a SCs senescent phenotype in Duchenne muscles. a Co-immunostaining of regenerative fiber, eMHC-positive (green) and Laminin (red) of Ctr and DMD muscles at different time points. Scale bar = 20 μm. b Quantification of the number of regenerative fibers, normalised on the total amount of fibers. A t-test was performed between 1–2 years and > 9 years old DMD groups. c Representative co-immunofluorescence of Pax7, Ki67 and Laminin in Ctr Quadriceps (Quadri, 7 years), DMD (Quadri 6 years) and in DMD dorsal muscles (Dorsal, 18 years). Yellow arrows point MuSCs (Pax7+). Nuclei are counterstained with DAPI (blue) (scale bar = 20 μm). d Quantification of the total number of Pax7+ cells on the total amount of fibers in DMD and control muscles at different time points. e Quantification of the number of active MuSCs (Pax7-Ki67 double positive) on the total number of MuSCs in DMD and Ctr muscles at different age. f Quantification of Pax7-P16 double positive MuSCs. g Quantification of Pax7-γH2AX double positive MuSCs. P-values were calculated by Mann–Whitney tests comparing control and DMD groups within the same age range