Fig. 2

Muscle substitution and increased number of FAPs. a Sirius red staining on Control (Ctr) and DMD biopsies at different time points (scale bar = 50 μm). b Quantification of the fibrotic red-stained area in Ctr and DMD muscles on the total surface of the section (mm²). c Quantification of fat-tissue infiltration (Bodipy stained area on mm²) in Ctr and DMD biopsies at different time points. d Representative immunofluorescence for PDGFRα-positive cells (red) and Laminin (green) in Ctr and DMD quadriceps at 1 year. Nuclei were counterstained with DAPI (blue) (scale bar = 20 μm). e Quantification of the number of PDGFRα-positive cells on mm² in Ctr and DMD biopsies at different time points. P-values were calculated by Mann–Whitney tests comparing control and DMD groups within the same age range