Fig. 2

Mushroom body lobes (MBLs) show age-related, region-specific TDP-43^WT accumulation and axonal fragmentation. a Illustrations of MBLs targeted by TDP-43^WT OE and observed changes with age (i, intact in young flies; iv, increasingly punctate TDP-43 YFP by middle-age; vii, axonal thinning and evidence of degeneration specifically in the γ lobe). These are presented alongside morphology of young (ii, iii,) middle-aged (v, vi) and old (viii, ix) control flies expressing membrane-bound RFP (mCD8 RFP) or cytoplasmic YFP. b Over expression of TDP-43^WT YFP in MBNs results in axonal localization of TDP-43 in young adult flies (iii) and dystrophic neurites in middle-aged (v) and old (viii) flies. c Signal intensity of mCD8 RFP with age in γ (left) and α/β (right) lobes. d Changes in TDP-43^WT YFP particle size with age in γ (left) and α/β (right) lobes. Data for TDP-43^G298S presented in Fig. 2-supplement 1. Scale bar = 25 µm * = P < 0.05; ** = P < 0.01; *** = P < 0.001