Fig. 1

TDP-43^WT overexpression in Kenyon cells results in cytoplasmic mis-localization and age-dependent cell loss. Illustrations in a, b, and c correspond to approximate plane of imaging for Kenyon cells (MBNs) in a’ larval, b’ young adult, and c’ old adult brain. a’ Nuclear localization of human TDP-43^WT YFP in the larval MBNs. b’ Young adult flies (1–3 days) contain cells with TDP-43^WT YFP in the cell body and cells showing nuclear loss of TDP-43^WT YFP (adjacent, double arrows as compared with single arrow). c’ Old adults (50–55 days) have fewer cells with nuclear depletion but concurrent loss in total number of TDP-43^WT positive neurons (quantified in e and f). Stacked, double arrows indicate a cell that has a signal intensity ratio > 1.0 due to punctate TDP-43 signal in the nucleus. Magenta asterisks indicate cells where nuclear depletion can be detected visually. d Histograms showing distribution of TDP-43^WT YFP signal intensity ratios (cell nuclei to whole cell) shift with age. Arrows correspond to measurements taken from cells shown in figure inset taken from b’, c’. e Age-specific TDP-43^WT YFP signal intensity ratios. f Decrease in TDP-43^WT YFP positive cells from young to old adults. SS01276 > YFP used as age-matched controls. Larval cell numbers provided for reference, but not used in statistical comparisons. Data for TDP-43^G298S YFP presented in Fig. 1-supplement 2. Scale bars = 50 µm. ** = P < 0.01; *** = P < 0.001