Fig. 1
From: A toxic gain-of-function mechanism in C9orf72 ALS impairs the autophagy-lysosome pathway in neurons
C9orf72 MNs display impairment of lysosomal trafficking along neurites. a Example kymograph from 40-day-old C9orf72 and isogenic control MNs loaded with Lysotracker Red. Tilted and straight vertical lines represent moving and stationary lysosomes respectively. Scale bar, 30 µM. b Quantification of the number of stationary lysosomes in proportion to neurite length (in pixels, px). c Quantification of the number of motile lysosomes in proportion to neurite length (in pixels, px). d Quantification of the percentage of motile lysosomes that pause or stop during their transport. Data represent mean ± SEM; data are pooled from three independent differentiations, and each dot represents a neurite (n = 180 neurites for all conditions). Statistical significance was assessed by one-way ANOVA and Tukey’s multiple comparison tests (b–d); *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001