Fig. 6

ACE2 levels are not altered in a model of AD, the 3xTg-AD mouse. A Determination of ACE2 levels by Western immunoblotting in brain homogenates from NonTg and 3xTg-AD mice aged 4, 12, and 18 months. No difference was observed in TBS-soluble and detergent-soluble ACE2. B In brain microvessel-enriched fractions from NonTg and 3xTg-AD mice aged 6, 12, 18 months, and in 18-month-old animals fed either a control diet or a high fat diet, no difference was observed in microvascular ACE2 levels. ACE2 levels in the mouse brain are not influenced by age or a diet that exacerbates AD-like neuropathology. Examples were taken from the same immunoblot experiment, and consecutive bands loaded in random order are shown. Actin and cyclophilin B are shown as loading controls. Data are represented as mean ± SEM. Statistical analysis: Kruskal–Wallis, ns, non-significant. ACE2 Angiotensin-Converting Enzyme 2, AD Alzheimer’s disease, Non-Tg,/NT Non-transgenic mice, 3 × 3xTg-AD mice, CD/C Control diet, CyB cyclophilin B, HFD/H high-fat diet, O.D. optical density, SEM Standard error of the mean, TBS Tris-Buffered Saline