Fig. 3

ACE2 protein in TBS-soluble and microvascular protein fractions show opposite relationships with detergent-soluble ACE2 when correlating with AD markers. Levels of ACE2 were investigated for their associations with several AD-relevant variables in human parietal cortex. Soluble and insoluble proteins were quantified in TBS-soluble and detergent-insoluble (formic acid) fractions, respectively, whereas BBB markers were measured in microvascular fractions [14, 15, 81, 82]. Linear regressions were performed to generate coefficients of determination (r²). Heat-map of hierarchical clustering analysis of correlation coefficients shows partial correlation analyses with antemortem evaluation, neuropathological markers and BBB markers. The significance of the correlation (inverse is highlighted in blue, positive in red) between two elements is entered in the associated box. Graph examples are shown in Figure S4. Statistical analysis: Coefficient of determination *p < 0.05, **p < 0.01 and ***p < 0.001. All proteins presented in these heat-maps were determined by western blot analysis except Aβ peptides, which were quantified using ELISA. Total soluble and insoluble Tau was detected using Tau (640–680) antibody. AD2 antibody binds to Tau phosphorylated at S396. Phosphorylated TDP43 antibody binds pSer409/410. ABCB1 ATP Binding Cassette Subfamily B Member 1, ACE2 Angiotensin-Converting Enzyme 2 ANPEP Aminopeptidase N, BBB Blood–brain barrier, BACE1 Beta-Secretase 1, CD31 or PECAM1 Platelet endothelial cell adhesion molecule, LRP1 Low density lipoprotein receptor-related protein 1, PDGFRβ Platelet Derived Growth Factor Receptor Beta RAGE Receptor for Advanced Glycation Endproducts, αSMA alpha smooth muscle actin, TDP-43 TAR DNA binding protein 43, TBS Tris-Buffered Saline