Fig. 6

NLRP3 inhibition-induced protection against IL-1β upregulation in mouse breast cancer brain metastasis. a Representative immunofluorescence micrographs (maximum intensity projections of z-stacks) showing the expression of IL-1β and GFAP in the brains of mice inoculated with 4T1-tdT TNBC cells (tumor-injected) or Krebs–Ringer solution (control) and treated with vehicle (DMSO in PBS) or 10 mg/kg MCC950 7 days after the injection of the tumor cells. b Quantitative analysis of colocalizing GFAP- and IL-1β-positive pixels compared to all pixels. Graphs represent the average ± SEM (N = 3 independent experiments, n = 5 sections/animal, 8 ROIs/section). ***P ≤ 0. 01 compared to “control, vehicle”, ^##P ≤ 0.01 compared to “tumor-injected, vehicle”, respectively (ANOVA and Fisher’s LSD post hoc test). ROI = region of interest