Fig. 5

Priming and activation of the NLRP3 inflammasome in the brains of mice bearing TNBC cells. a Expression of the Nlrp3 and Il1b genes in the brains of mice 7 days after intracarotid injection of 4T1-tdT cells treated with vehicle (DMSO in PBS) or 10 mg/kg MCC950. Graphs represent the fold change (normalized to GAPDH), average ± SEM (N = 3 independent experiments, each performed in triplicate). *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001, ****P ≤ 0.0001 compared to “control side, vehicle”, ^#P ≤ 0.05, ^####P ≤ 0.0001 compared to “injected side, vehicle”, respectively (ANOVA and Fisher’s LSD post hoc test). b Representative western blot showing the expression of active IL-1β in the respective hemispheres. c Quantitative analysis of active IL-1β levels compared to β-actin expression, as assessed by western blot. Graphs represent the average ± SEM (N = 3 independent experiments). **P ≤ 0.01 compared to “control side, vehicle”, ^##P ≤ 0.01 compared to “injected side, vehicle”, respectively (ANOVA and Fisher’s LSD post hoc test)