Fig. 2

Upregulation of inflammasome components in peritumoral astrocytes in mouse TNBC brain metastases. a Representative immunofluorescence micrographs (maximum intensity projections of z-stacks) showing time-dependent upregulation of IL-1β expression and astrocyte reactivity in mouse TNBC brain metastases. At 5 and 7 days, 77% and 79% of cytoplasmic IL-1β-positive pixels colocalized with GFAP, respectively. At 5 and 7 days, 7% and 28% of GFAP-positive pixels were IL-1β-positive. b Representative maximum intensity projections of z-stacks showing expression of NLRP3 in peritumoral astrocytes 7 days after inoculation of the tumor cells. Ninety-five percent of NLRP3-positive pixels colocalized with GFAP. Red: 4T1-tdT, in all figures