Fig. 4
From: Rapid isolation of intact retinal astrocytes: a novel approach
RT-qPCR and immunohistochemistry of astrocytic enrichment from pull-off and pull-off plus immunoprecipitation in microbead-injected mice. A: RT-qPCR results from microbead-treated animals showing fold-change enrichment of markers of astrocytes (GFAP), Müller cells (Cralbp), inner retinal neurons (Pax6), and outer retinal neurons (NeuroD1) in pull-off (PO) and pull-off plus immunoprecipitation (PO + IP), relative to whole retina control. B–E: Immunostaining of pull-off isolated tissue from microbead injected mice. Microbead treatment did not interfere with the isolation process, and HA continues to colocalize with astrocytes as in naïve mice. Scale bar in all panels = 100 µm