Fig. 6

Multiplexed staining of glia in relation to vascular profiles and analysis of Vascular Phenotypes A sequential multiplexed staining and analysis, known as QUIVER, was employed on human FFPE tissue. The procedure started with the staining for CD34 to visualize multiple vascular profiles (MVP) and single vascular profiles (SVP) in the same case (A). This step utilized a permanent chromogen to preserve the staining throughout each subsequent round. Subsequent staining rounds were performed for ferritin (B, F), GFAP (C, G), and IBA1 (D, H), sequentially, using a removable chromogen. Post-deconvolution of single-channel IHC images, merged pseudo-fluorescent images were generated for MVP (I, J) and SVP (K, L). Cell count data was produced using the object colocalization algorithm in the HALO software, and digital markup (J and L). The color labels correspond with the representative color in the markup image and merged image. Scale bars = 50 μm