Fig. 2

Schematic of MVP quantification. The photograph is of a CD34 stained tissue from the frontal cortex (Broadmann area 9) of an 84 year-old female. Using Aperio ScanScope digital slide scanner accompanying image analysis software, the grey matter area was outlined (green-black line). Next, the grey matter area was manually scanned for MVPs which were marked by a counter (pink crosses). The total # of MVPs and the grey matter area for each case were used to calculate an MVP density. Scale bar = 5 mm