Fig. 7

IFNAR deficiency reduces T cell accumulation following TBI. T cell accumulation was quantified using flow cytometry. (A) Representative flow cytometry gating strategy. TBI increased the total number of CD4+ T cells (B) and of CD8 + T cells (C) at 3 and 10 DPI in WT mice; IFNAR KO mice showed no difference in T cell numbers after TBI compared to the IFNAR KO control, and a significant decrease in total CD8 + T cells compared to WT TBI at 3 and 10 DPI. Error bars depict mean ± SEM. Statistical analysis performed by two-way ANOVA with Tukey’s test for multiple comparisons. * p < 0.05, n = 4 for control groups, n = 4–6 for TBI groups. (D-H) Representative IHC for CD8α demonstrating localization of CD8 +  T cells (arrowheads) in the cortex (E), corpus callosum (F), hippocampus (G), and thalamus (H) at 7 days following WT TBI. Scale bar = 50 μm. WT control sections not shown. n = 4 mice/group