Fig. 6

IFNAR deficiency reduces acute monocyte accumulation following TBI. Leukocyte cell accumulation was quantified using flow cytometry. (A) Representative flow cytometry gating strategy. (B) Total microglial numbers were not affected by genotype or brain injury. (C) WT TBI resulted in an increase in the total population of CD45+ high leukocytes. More specifically, the neutrophil population was not significantly altered by genotype of brain injury (D). Ly6C+ and Ly6C- monocytes were increased at 3 DPI following WT TBI, while monocyte accumulation in IFNAR KO TBI mice were not significantly different than genotypic controls (E and F). Error bars depict mean ± SEM. Statistical analysis performed by two-way ANOVA with Tukey’s test for multiple comparisons. * p < 0.05, n = 4 for control groups, n = 4–6 for TBI groups