Fig. 5

IFNAR deficiency reduces tissue and microglial MHC class I molecule expression at 7 and 31 days post-TBI. Expression of H2- K1 (A) β2m (B) and Tap1 (C) were evaluated by qPCR in ipsilateral hippocampus at 7 and 31 DPI in WT and IFNAR KO mice. Data are expressed as fold change in gene expression relative to WT control. Statistical analysis with two-way ANOVA with Tukey’s test for multiple comparisons. *p < 0.05, n = 5 mice/group. Evaluation of TBI-induced microglial H2-K1 expression using dual in-situ hybridization and IHC: H2-K1 (green), IBA1 (red), DAPI (blue). 7 DPI WT mice (D-H) had greater H2-K1 expression and co-localization with a microglial marker, IBA1, than IFNAR KO mice (I-M) in all regions examined. Scale bar = 50 μm. WT and IFNAR KO control images are not shown. Representative images from n = 4–5 mice/group