Fig. 3

IFNAR deficiency decreases Cxcl10 gene expression throughout the ipsilateral forebrain. Evaluation of TBI-induced Cxcl10 expression using RNAscope in-situ hybridization: Cxcl10 (green), DAPI (blue) 7 days post injury. Representative images demonstrating TBI-induced Cxcl10 expression in WT mice (A-E) and IFNAR KO mice (F-J). Scale bar = 20 μm. WT and IFNAR KO control sections are not shown. (K-N) Quantification of number of Cxcl10 + cells in WT TBI and IFNAR KO TBI mice. Error bars depict mean ± SEM. Significance determined by two-tailed unpaired t-tests for normally distributed data and Mann-Whitney U tests for non-normally distributed data comparing WT TBI vs. IFNAR KO TBI in each region. *p < 0.05 WT TBI compared to IFNAR KO TBI, n = 4–5 mice/group