Repeat length of C9orf72-associated glycine–alanine polypeptides affects their toxicity

Table 1 List of primers

Method	Primer name	Primer sequence	Purpose
Fly genotyping	JOL36	AGCAACCAAGTAAATCAACTGCA	Verification of untagged and tagged polyGA lines
	JOL37	TGTCCAATTATGTCACACCACAG
Cloning	JOL124	ATATGCGGCCGCCGGTAGTGGAAGTGGTAGTATGGTGAGCAAGGGCGAGGAGCTGTTCAC	Generation of the pUAS T-GFP-C plasmid
	JOL125	AAAAGGTACCTCACTTGTACAGCTCGTCCATGCGGAGAGTGAT	Generation of pUAS T-GFP-C plasmid
	SOL565	ATGCGGCCGCTTGCCCCCGATCACAGAGTG	Generation of the pUAS RPN11 plasmid
	SOL566	ACTCTAGAAGGGAAATTGGTTTGAGGAGAGC	Generation of the pUAS RPN11 plasmid
Q-RT-PCR	JOL171	ACCAGCAACCAAGTAAATCAAC	Q-RT-PCR for UAS transgenes [41]
	JOL172	ATTCCCAATTCCCTATTCAGAG	Q-RT-PCR for UAS transgenes [41]
	SOL268	ATATGCTAAGCTGTCGCACAAATGG	Q-RT-PCR for Rpl32 [75]
	SOL269	GATCCGTAACCGATGTTGGGCA	Q-RT-PCR for Rpl32 [75]
	JOL251	ACCATGATAGCCAGGGTCAT	Q-RT-PCR for Sec22
	JOL252	GCATCTTAGCCTGGTTTTGG	Q-RT-PCR for Sec22
	JOL255	ACTCTCTCCGACAAGCGACT	Q-RT-PCR for Tango1
	JOL256	AAGGATATCAGCCCCTCACC	Q-RT-PCR for Tango1

ISSN: 2051-5960