Fig. 7

Down-regulation of Tango1 reduces GA400 toxicity. A Climbing ability of female flies that co-express GA400 and Tango1 RNAi under the control of the elav-GS driver. Climbing indices are represented as box plots and the mean is indicated by +. Circles indicate individual flies. Down-regulation of Tango1 significantly improved climbing ability of GA400-expressing flies (Two-Way ANOVA + Bonferroni’s multiple corrections test; n = 34–44 flies; ****P < 0.0001). B Survival curves of female flies that co-express GA400 and Tango1 RNAi or Sec22 RNAi under the control of the elav-GS driver. Down-regulation of Tango1, but not of Sec22, significantly extended lifespan of GA400-expressing flies (log-rank test + Bonferroni’s multiple corrections test; n = 150 female flies for elav-GS/UAS-GA400 and elav-GS/UAS-GA400, UAS-Sec22-RNAi, n = 90 for elav-GS/UAS-GA400, Tango1-RNAi; P < 0.0001). The same GA400 control survival curves are shown in B and Additional file 12: Fig. S12D-F. C Western blot analysis of GA and p62 protein expression in fly heads expressing GA400 in combination with Tango1-RNAi under the control of elav-GS for 5 days. Quantification of D HMW GA and E p62 protein levels from the western blot analysis in C. There was no significant difference in GA400 or p62 levels when Tango1 RNAi was co-expressed with GA400 (One-way ANOVA + Tukey’s multiple comparisons test; n = 4 replicates of 20 female fly heads; **P < 0.01, ***P < 0.001, ****P < 0.0001)