Fig. 6

GA400 expression up-regulates ER-Golgi markers in the fly brain. A Schematic overview of ER-Golgi proteins identified by the proteomics analysis. B-J ER-Golgi protein levels measured by mass spectrometry-based proteomics in the brain of female flies expressing GA100, GA200 or GA400 for 5, 15 or 40 days under the control of the elav-GS driver. Shown are z-score-normalized protein levels for B-C Sec16 (B: Isoform X2JEY9, C: Isoform A8JUU3), D Sec23, E–F Sec24 (E: Isoform AB, F: Isoform CD), G Sec13, H Sec31, I Sec22, and J Tango1. While induction of GA400 for 5 days was sufficient to significantly increase the levels of all shown ER-Golgi proteins, significant effects for GA100 and GA200 were only observed after prolonged expression (One-Way ANOVA + Tukey’s multiple corrections test; n = 3–4 replicates of 25 fly brains; *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001). Cartoon in A was generated using biorender