Fig. 4

PolyGA expression affects the fly brain proteome in a repeat length-dependent manner. A–F Mass spectrometry-based proteomics analysis of female fly brains expressing GA100, GA200 and GA400 under the control of the elav-GS driver. A–C VENN diagrams of the overlap of differentially expressed proteins in fly brains that express GA100, GA200 and GA400 pan-neuronally for 5 (A), 15 (B) or 40 (C) days. D VENN diagrams show the overlap of proteins that is differentially expressed between elav-GS/ + and GA400 flies at 15 days compared to those between GA100 and elav-GS/ + or GA200 and elav-GS/ + at 40 days of induction. A–D Up-regulated proteins (upper panel) and down-regulated proteins (lower panel). E–F Gene ontology (GO term) enrichment of (E) up-regulated and (F) down-regulated proteins in GA100, GA200 and GA400 brains after 5, 15 and 40 days of transgene induction. Only the most significant GO terms (p.adjust < 0.05 after Bonferroni correction) are displayed