Fig. 1

The effect of repeat length on GA aggregation, subcellular localisation and p62 level. A, B Western blot analysis of fly heads expressing GA100, GA200 and GA400 in adult neurons for A 8 h, B 5 and 15 days under the control of the inducible elav-GS driver. An anti-GA antibody was used for immunodetection. C Representative images of adult fly brains expressing GA100, GA200 or GA400 for 5 days under the control of the elav-GS driver. Brains were stained with anti-GA and anti-p62 antibodies, and with DAPI to stain nuclei. Images from the antennal lobes are shown, which allow distinguishing neuronal somas from axons. Scale bar: 10 µm. D Western blot of fly heads expressing GA100, GA200 and GA400 for 5 and 15 days under the control of the elav-GS driver. Extracts were probed with an anti-p62 antibody. E Quantification of p62 protein levels based on the immunoblot in D (Two-way ANOVA + Tukey’s multiple comparisons test; n = 3 sets of 20 fly heads; genotype: ****P < 0.0001; age: ****P < 0.0001; interaction of age and genotype: P > 0.05)