Fig. 8

Imipramine prevents plaques deposition and neuronal cell death. A Representative photomicrographs of sagittal brain sections imaging the cortex and subiculum of 5xFAD labeled Aβ plaques in green. All photomicrographs were exposed and processed identically. Scale bar represents 500 µM. B Immunofluorescence quantification of plaques measured by plaques counts/mm² and in C Plaques size area. D Western blot analysis of hippocampal homogenate stained with 6E10 antibody showing APP and Aβ levels normalized to β-actin and the ratio of amyloid Aβ/APP. Western blot membranes are shown in Additional File 1. E Representative photomicrographs and quantification of FluoroJadeC positive cells (Green staining) in the subiculum. Box and Whiskers plot of 4–5 animals per experimental condition, Unpaired t-test was applied (*p < 0.05, **p < 0.01). F Quantification of 3-Nitrotyrosine relative levels in pre-frontal cortex of WT, 5xFAD vehicle and Impramine groups. Bar graphs represent the mean ± SD of N = 5/group. One-way ANOVA, LSD (*p < 0.05, **p < 0.01)