Fig. 5

Imipramine prevents astrocytic ceramide formation in vivo. A E Quantification of C18:0, C24:0, C26:0 and C26:1 Cer by mass spectrometry in the hippocampus. Bar graph represents average ± SD with each N = 4–8/group. Two-way ANOVA followed by LSD postdoc analysis (*p < 0.05, **p < 0.01). B Representative photomicrographs of Iba 1 (Magenta), GFAP (Red), Cer (Green), and Hoechst (Blue) in the subiculum region of 5xFAD animals treated with vehicle or Imipramine (Scale bar 10 µm). C Box and Whiskers plot of correlation analysis of GFAP and Iba1 staining with Cer immunolabelling represented as Mander’s overlap values (Vehicle N = 5 and Imipramine N = 5 for groups). Unpaired t-test (**p < 0.01)