Fig. 2

Inhibition of A-SMase prevents astrocytic Cer formation. A Schematic diagram showing treatment of astrocytes with microglia-conditioned media. B Quantification of GFAP and C3b by western blot analysis in astrocytes after 24 h incubation with conditioned media from microglia (Western blot membranes are shown in Additional File 1.). Bar graphs represent average ± SD of N > 3/group. One-way ANOVA, Dunnett’s posthoc testing (*p < 0.05, **p < 0.01, ***p < 0.001). C Quantification of C14:0, C16:0, C18:0, C18:1, C20, C20:1, C22:0, C22:1, C24:0 and C24:1 by mass spectrometry of astrocytes after incubation with conditioned media from microglia. Bar graph represents average ± SD with each N = 3 group. One-way ANOVA, Dunnett’s posthoc testing (*p < 0.05, **p < 0.01, ****p < 0.0001