Fig. 4

Trisomy of Hsa21 does not alter the localisation of cystatin B (CSTB) to the lysosome. a–c Total cellular proteins from disomic and trisomy 21 human primary fibroblasts were separated into cytosolic and nuclear fraction and the abundance of CSTB quantified by western blot, LSD1 was used as a marker of the nuclear fraction. b Relative CSTB abundance was increased by trisomy 21 (Mann–Whitney U test p = 0.0286) but the nuclear/cytosolic CSTB ratio was not altered (Mann–Whitney U test p = 0.8857). d, e Colocalisation of CSTB (cyan) with LAMP1 (magenta) did not differ between disomic (Pearson’s Correlation R = 0.555) and trisomy 21 cells (Pearson’s Correlation R = 0.582) (Mann–Whitney U test p = 0.8571). For d and e, individual data points are technical means of 3 technical replicates for n = 4 disomic and n = 3 trisomy 21, independent cell lines, error bars SEM. *p < 0.05