Fig. 3

Tau pathology of the present case. a, d, g, j Numerous p-tau-positive neuronal and glial inclusions, as well as threads, are visible in the middle temporal gyrus (a), precentral gyrus (d), amygdala (g), and substantia nigra (j). These inclusions and threads are positive for RD4 (c, f, i, l) but negative for RD3 (b, e, h, k). d–f The insets, which show Betz cells, are high magnification of squares in each panel. Betz cells are positive for p-tau and RD4 but negative for RD3. Immunohistochemistry of p-tau (a, d, g, j), RD3 (b, e, h, k), and RD4 (c, f, i, l). Scale bar in (a) represents 200 µm (a, d–g) and 100 µm (b, c, h–l). m Immunoblot analyses of sarkosyl-insoluble fractions prepared from the brain of this patient and tauopathy patient references. Sarkosyl-insoluble full-length tau (64 kDa and 68 kDa) and C-terminal fragments (33 kDa and 37 kDa) were detected using the T46 antibody (residues 404–441). n Immunoelectron microscopy of sarkosyl-insoluble fractions extracted from the brain. An electron micrograph shows twisted filaments that are positive for the AT8 antibody