Fig. 4

Hypoxia induced expression and secretion of VEGFA in Oli-neu cells. (A) The expression of Vegfa mRNA was constantly increased in hypoxic Oli-neu cells compared to normoxic cell expression from 12 to 48 h, with a peak increase after 24 h. (B) Hypoxic Oli-neu cells significantly secreted more VEGFA protein into conditioned media 24 h after exposure to hypoxia compared to normoxia. (C) Oli-neu cells exposed to hypoxia did not significantly increase mRNA expression of Hif1α after 24 h exposure, while Epas1 (aka Hif2α) was significantly increased compared to normoxic cell expression. (D) inhibition of Hif1α and/or Epas1 results in inhibition of hypoxia mediated increase in Vegfa expression. (E) Both Claudin-5 (Cldn5) and (F) Occludin (Ocln) mRNA expression was significantly decreased in brain EC treated with hypoxic OPC derived CM. Mean ± SEM; ns = not significant, **p < 0.01, ****p < 0.0001, vs 21%; unpaired student t-test or one-way ANOVA with Tukey’s multiple comparisons test