Fig. 3
Hypoxia induced the expression of 417 DEGs. (A) Hypoxia (2% O2) induced the expression of 417 differentially expressed genes (DEG) compared to normoxia (21% O2) in OPC, of which 256 were upregulated and 171 downregulated. (B) Volcano plot showing the 417 DEG in hypoxic OPC with genes that were downregulated in red, and upregulated genes in green (C) DAVID enrichment analysis shows enrichment of pathways including HIF-1 signalling pathway, positive regulation of cell migration, and angiogenesis. Terms were considered significant with an EASE score < 0.05 and FDR < 0.05. (D) VEGFA was identified as a potential key mediator in these pathways and secreted protein concentration in the hypoxia exposed conditioned medium was increased compared to normoxia. Mean ± SEM; ****p < 0.0001, vs 21%; unpaired student t-test