Fig. 2

BCAS led to an increase in hypoxic OPC in deep cortical regions after 7 days. (A) Immunolabeling for MBP and MBP clone SMI94 in the corpus callosum. Scale bar, 50 µm. (B) Quantification of MBP integrity, (C) MBP negative area, and (D) Myelin degradation, quantified by intensity signal and the number of hyperintense foci, respectively, was not statistically different between BCAS and Sham mice 7 post-operative days. (E) immunolabeling for Olig2, CC1, and Pimonidazole-Pacific blue in the deep cortical regions. Scale bar, 50 µm. (F) A significant increased hypoxic cell density was observed in the deep cortical regions of BCAS animals compared to Sham animals. (G) BCAS mice also showed an increased number of hypoxic OPC in these regions compared to Sham animals. Mean ± SEM; *p < 0.05, **p < 0.01; unpaired student t-test or Mann–Whitney U-test