Fig. 2
From: Human Alzheimer’s disease reactive astrocytes exhibit a loss of homeostastic gene expression
Protoplasmic astrocytes exist within a spectrum of reactivity. a Protoplasmic and fibrous astrocyte nuclei plotted using partition-based graph abstraction (PAGA). b SLC1A2 expression in protoplasmic astrocytes. c CD44 expression in fibrous astrocytes. d RNA trajectory analysis was used to calculate pseudotime values for astrocyte nuclei. e VIM, a known reactive astrocyte gene, increased along pseudotime. f NRXN1, a known homeostatic astrocyte gene, decreased along pseudotime. g Plot of linear mixed effects model coefficients for modeling nuclei pseudotime as a function of donors’ pathologic, genetic, and demographic features (*p = 0.040). Error bars represent standard error