Fig. 1
From: Human Alzheimer’s disease reactive astrocytes exhibit a loss of homeostastic gene expression
Identifying protoplasmic and fibrous astrocyte clusters. a t-distributed stochastic neighbor embedding (t-SNE) projection of 122,606 sequenced nuclei across 15 normal, pathologic aging, and Alzheimer’s disease human brains from a previously published single-nucleus RNA sequencing dataset [50] with astrocyte nuclei colored in blue. b–d Expression feature plots of known astrocyte-specific marker genes. e–i Uniform Manifold Approximation and Projection (UMAP) plots and j Sankey diagram of the 15,529 astrocyte nuclei as they were clustered at varying resolutions. k Expression feature plots of known marker genes for protoplasmic astrocytes, fibrous astrocytes, neurons, and oligodendrocytes at the four cluster resolution. l Heatmap showing cluster annotations based on differential gene expression