Table 3 Summary of antibodies and antigen retrieval methods used in study
Key resources table | |||||
|---|---|---|---|---|---|
Primary antibodies | Identifier | Specificity | Antigen retrieval for IHC | Source | References |
Anti-αSyn pS129, Mouse | 81A RRID: AB_2819037 | α-syn at pSer129 | Water w/0.05% Tween, heat bath | B. Giasson University of Florida College of Medicine; Florida; USA | [50] |
p62/ sequestresome-1, Rabbit | p62 | Sequestresome1 | Water w/0.05% Tween, heat bath | ProteinTech, Rosemont, IL | [24] |
Anti-αSyn, Mouse | 2H6 | αSyn (2–21) | Water w/0.05% Tween, heat bath | B. Giasson University of Florida College of Medicine; Florida; USA | [10] |
Anti-αSyn, Mouse | 3H19 | αSyn (110–119) | DAKO Target Retrieval Solution, heat bath/70% formic acid | B. Giasson University of Florida College of Medicine; Florida; USA | [29] |
Anti-αSyn cleaved at 103, Mouse | 2G5 | αSyn (x-103) | Formalin incubation/DAKO Target Retrieval Solution, heat bath/70% formic acid | B. Giasson University of Florida College of Medicine; Florida; USA | [20] |
Anti-αSyn cleaved at 114, Mouse | 1A2 | αSyn (x-114) | DAKO Target Retrieval Solution, heat bath/70% formic acid | B. Giasson University of Florida College of Medicine; Florida; USA | [40] |
Anti-αSyn cleaved at 122, Mouse | 10A4 | αSyn (x-122) | DAKO Target Retrieval Solution, heat bath/70% formic acid | B. Giasson University of Florida College of Medicine; Florida; USA | [20] |
Anti-αSyn cleaved at 125, Mouse | 5C1 | αSyn (x-125) | DAKO Target Retrieval Solution, heat bath/70% formic acid | B. Giasson University of Florida College of Medicine; Florida; USA | [20] |
Anti-αSyn cleaved at 129, Mouse | 2G7 | αSyn (x-129) | DAKO Target Retrieval Solution, heat bath/70% formic acid | B. Giasson University of Florida College of Medicine; Florida; USA | [20] |