Fig. 8
Medullary αSynΔC-114 inclusion pathology is positively correlated with onset of motor symptoms in APOE ε4+/+/M83+/− mice. (a) Graph summarizing burden of αSynΔC-114 positive inclusions in the spinal cord (SC), medulla (MY), pons, midbrain (MB), hypothalamus (HY), thalamus (TH), cortex (CTX) and striatum (STR) of PFF-injected M83+/−, APOE ε4+/+/M83+/− and APOE ε3+/+/M83+/− mice depicted as mean with SEM. (b) Semi-quantification and representative images of αSynΔC-114 immunoreactivity in the medulla with correlational analysis between αSynΔC-114 immunoreactivity and d.p.i. for each cohort. (c) Semi-quantification and representative images of αSynΔC-114 immunoreactivity with correlational analysis between αSynΔC-114 immunoreactivity in the midbrain and d.p.i. for each cohort. M83+/− horizontal line. Results were analyzed using 2-way ANOVA and corrected using Tukey’s multiple comparisons test; data represent means +/− SEM. Pearson correlation coefficients between αSynΔC positivity and d.p.i. were computed with the assumption that data are sampled from Gaussian distribution. Pearson r and two-tailed p-values are indicated on graph. Simple linear regression is shown with dotted lines depicting 95% confidence intervals. n.s. not significant; *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. Scale bar = 100 μm